Liquid chromatography coupled to tandem mass spectrometry for detecting ten allergens in complex and incurred foodstuffs

摘要

Highlights?Selection of abundant and specific peptides in unprocessed and processed matrices.?An efficient UHPLC–MS/MS method for routine laboratories, using a single protocol.?Sensitive detection of 10 allergens by UHPLC–MS/MS in incurred foodstuffs.?Mass spectrometry: a method complementary to the ELISA for allergen detection.?Applicability of the method to detecting allergens in complex samples.AbstractFood allergy is a considerable heath problem, as undesirable contaminations by allergens during food production are still widespread and may be dangerous for human health. To protect the population, laboratories need to develop reliable analytical methods in order to detect allergens in various food products. Currently, a large majority of allergen-related food recalls concern bakery products