Effects of GPTMS concentration and powder to liquid ratio on the flowability and biodegradation behaviors of 45S5 bioglass/tragacanth bioactive composite pastes

摘要

Injectable composite pastes were prepared using melt-derived 45S5 bioactive glass and tragacanth crosslinked by (3-glycidyloxypropyl)trimethoxysilane (GPTMS). The effect of powder to liquid ratio (P:L= 1.0:2.0-1.0:2.5) and GPTMS/tragacanth ratio (0.0-1.5) on the injectability, swelling behavior, rheology, bioactivity, and cellular behavior of the pastes was investigated. Based on the results, the apparent stability and consistency of the pastes increased upon crosslinking by GPTMS. Due to the increased interactions between tragacanth and glass, a hysteresis loop with larger area was formed in the presence of GPTMS. With increase of GPTMS:tragacanth ratio from 0 to 1.5, the swelling percent dropped from 24.65 to 16.25% after 24h and the degradation percent also went down from 27.89 to 9.11% after 21days in the simulated body fluid. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed a drop in the optical density of MG63 osteoblasts up to 30.07% after exposure to the GPTMS-crosslinked composite pastes for 3 days. However, the number of viable cells gradually increased in the presence of the pastes and the cell morphology remained unchanged over time. (c) 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47604.