Phase behavior, in vitro drug release, and antibacterial activity of thermoresponsive poloxamer-polyvinyl alcohol hydrogel-loaded mupirocin nanoparticles

摘要

This research was designed to develop thermoresponsive poloxamer (P407)-polyvinyl alcohol (PVA) hydrogels to deliver mupirocin nanoparticles for wound healing. The mupirocin nanoparticles containing drug and gelatin or poly (acrylic acid) were prepared by spray drying. The hydrogel phases were evaluated by small-angle X-ray scattering. An in vitro drug release study was performed and the antibacterial activity of mupirocin nanoparticles-loaded hydrogel (MLH) was evaluated. Fourier transform infrared and proton nuclear magnetic resonance spectrum spectra of the mupirocin nanoparticles indicated a weak interaction between mupirocin and the carriers of carbopol and gelatin. The mupirocin molecules were surrounded by the carrier molecules. The MLH appeared to exhibit single diamond (Fd3m) phase behavior similar to P407 and the hydrogel base. The release of MLH in vitro indicated first-order kinetics (R-2 = .9839-.8868). The MLH showed lower minimum inhibitory concentrations and minimum bactericidal concentrations against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli than mupirocin ointment.