Selection of a DNA Aptamer against Zearalenone and Docking Analysis for Highly Sensitive Rapid Visual Detection with Label-Free Aptasensor

摘要

Contamination of feed with zearalenone (ZEN) presents a significant risk to animal health. Here, a visible, rapid, and cost-effective aptamer-based method is described for the detection of ZEN. After 8 rounds of SELEX (systematic evolution of ligands by exponential enrichment) with an affinity-based monitor and counter-screening process, the ssDNA aptamer Z100 was obtained, which had high affinity (dissociation constant = 15.2 +/- 3.4 nM) and good specificity. Docking analysis of Z100 indicated that noncovalent bonds (pi-pi interactions, hydrogen bonds, and hydrophobic interactions) helped ZEN to anchor in the binding sites. Finally, a label-free detection method based on gold nanoparticles and Z100 at 0.25 mu M was developed for ZEN determination. Excellent linearity was achieved, and the lowest detection limit was 12.5 nM. This rapid and simple method for ZEN analysis has high sensitivity and can be applied for on-site detection of ZEN in animal feeds.