Protective role of black berry juice against hepatotoxcity and reproductive toxicity of chlorpyrifos in male rats.

摘要

Chlorpyrifos (CPF) is causing different sorts of toxicity and black berry juice is a new species of Black berry juice and it is the first time to study its effect against the toxicity of CPF. Therefore, the present study is aimed to elucidate the possible protective effects of Black berry juice in alleviating the toxicity of chlorpyrifos on reproductive performance, testosterone levels, enzyme activities and lipids profile in serum of male rats. Animals were divided into four groups; one orally administered (o.d.) CPF at a dose of 9 micro g/kg b.w. for consecutive 80-days, Black berry juice group (50 mg/kg b.w.), Black berry juice and chlorpyrifos group and a control group. Results showed that there was a correlation between CPF administration and significant decrease of the sperm counts, spermatozoon survival and testosterone level as well as increase of sperm aberrations. CPF increased significantly the lipid profile and the levels of various serum liver marker enzymes. In contrast, co-administration of Black berry juice to CPF-treated rats restored almost most of these biochemical parameters to normal levels. On the other hand, CPF resulted in histopathological alterations in testes of male rats. However, pre-administration of Black berry juice to CPF-treated animals improved the testicular damage and alleviates the toxic effects of CPF on reproductive functions and neural functions in male rats.