Extractive spectrophotometric determination of tenofovir

摘要

Two simple, economical, precise, reliable and reproducible visible spectrophotometric methods (A and B) have been developed for the estimation of Tenofovir (TNF) in bulk as well as in Tablet formulations. The developed methods A and B are based on theformation of chloroform extractable complex of Tenofovir with Bromocresol green (Method A) and Erichrome (Method B), which shows absorbance maxima at 420 nm and 510 nm respectively. The absorbance-concentration plot is linear over the range of 25-250 mcg/ml for Method A, and 25-200 mcg/ml for Method B. The different experimental parameters affecting the development and stability were studied carefully and optimized. Results of analysis for all the methods were validated statistically and by recovery studies.