Geographical variation of Juglans regia L. in juglone content: rapid analysis using micro-plate reader

摘要

Results are presented of a study conducted to understand the variation in juglone content in leaves of J. regia in the provenance trial in Oxfordshire, UK, of this species from 18 geographical origins. However, only 12 provenances (10 from Kyrgyzstan and one each from Spain and France) were considered in this study because only these provenances had 25 or more individuals per provenance. For juglone content, 1.22% of the total variation was explained by differences between provenances and 77.96% by differences between trees within provenances. Variance component due to differences between provenance for juglone content was only 1.22 (%), which is less compared to 7-year height (14.16%) and 7-year collar diameter (14.54%). The overall mean juglone content in the trial was 357.0 mg/100 g dry weight. The minimum value of juglone content was 13.1 mg/100 g dry weight and the maximum value was 1556.0 mg/100 g dry weight. The mean juglone content was greatest for provenance Katar Yangak followed by provenance Ak Terek (both Kyrgyzstan) and the least for provenance Catalonia (Spain).