Isolation and Identification of a Phenol-Degrading Yeast JP-1 with High Activity and Its Degrading Characteristics and Kinetic

摘要

A yeast strain with high activity of phenol degradation was isolated from activated sludge of a wastewater treatment plant in a coking chemical factory, and identified as Candida tropicalis according to the analysis of VITEK-32 and its 18SrDNA gene sequence. The strain designated as JP-1 was able to grow on phenol as a sole carbon and energy source and it can degrade more than 90% of 1800 mg-L~(-1) phenol within 72h. The optimum pH was 6.0. Kinetic equation of phenol degradation was obtained by regression using MATLAB software. The parameters of Haldane equation were following: mu_(max)=0.387 1-hr~(-1), fc=4.20 mg-L~(-1), K_iK_i=465.6 mg-L~(-1). Cell grew was obviously inhibition by high concentration phenol and the inhibition strengthen increased with phenol increasing.