Preliminary analysis of cuprome ofAnabaena doliolum using two-dimensional gel electrophoresis

摘要

This study provides first-hand information on the initialcharacterization of copper-induced changes in theglobal proteome (hereafter called cuprome) of Anabaenadoliolum subjected to short- and long-term treatments.PD Quest analysis revealed that out of 215 protein spotsin the control, 79 showed alterations (26 up- and 36down-regulation, and 5 up- and 12 down-regulation res-pectively, after 24 and 168 h of Cu treatment) in their expression pattern. The short-term (24 h) and long-term(168 h) treatments induced respectively, 158 and 96proteins. Of the 158 newly induced proteins, 30 werefound to sustain the long-term treatment. In view ofthe appearance of two sets of proteins, there appears aneed to carry out short- and long-term proteomeanalysis for getting a holistic view of the proteome ofcyanobacteria subjected to abiotic stress.