Effect of insecticides and inhibitors on P-glycoprotein ATPase (M-type) activity of resistant pest Helicoverpa armigera.

摘要

P-glycoprotein (P-gp) was detected using C219 antibodies from the membrane preparation of the insecticide-resistant pest, H. armigera. This protein was partially purified and found to be a glycoprotein having ATPase [adenosinetriphosphatase] activity. Its molecular mass, as determined by SDS-PAGE, was 150 kDa. Monocrotophos, endosulfan, cypermethrin, fenvalerate and methyl parathion [parathion-methyl] stimulated P-gp ATPase activity. Orthovanadate at 1.5 micro M inhibited P-gp ATPase activity by 70%, whereas HgCl2 and PCMBS [p-chloromercuribenzene sulfonic acid] at 200 micro M inhibited the enzyme activity by 75 and 70%, respectively. EDTA and sodium azide inhibited P-gp ATPase activity by 20% at 0.5 and 2 micro M, respectively. Sodium molybdate (10 micro M) did not inhibit P-gp ATPase activity.