Fluorescence lifetime imaging of intracellular magnesium content in live cells Electronic supplementary information (ESI) available

摘要

The first detailed analysis for Mg cell imaging by visible FLIM is presented. We employed the Mg-sensitive fluorescent dye named DCHQ5, a derivative of diaza-18-crown-6 ethers appended with two 8-hydroxyquinoline groups, to perform fluorescence life-time imaging in control and Mg deprived SaOS-2 live cells, which contain different concentrations of magnesium. We found that the lifetime maps are almost uniform all over the cells and, most relevantly, we showed that the ratio of the amplitude terms is related to the magnesium intracellular concentration.