An acetophenothiazine-based fluorescence probe for multi-channel imaging of thiophenol with a large Stokes shift

摘要

By integrating a phenothiazine moiety as an electron donor and an acetyl moiety as the electron acceptor, a novel D-pi-A system dye, PTZAc, with a broadband emission spectrum (the full width at half maxima can reach to 123 nm) and a large Stokes shift (up to 217 nm) was developed. Based on this ideal platform, a novel 2,4-dinitrophenyl-ether type fluorescent probe, PTZAc-1, for thiophenol detection was fabricated. Upon addition of thiophenol, PTZAc-1 exhibited drastic fluorescence enhancement (over 26 folds intensity enhancement at 590 nm) and the corresponding detection limit was calculated to be 1.03 mu M. Significantly, the application of PTZAc-1 for intracellular thiophenol imaging in a multi-channel manner was successfully demonstrated in living RAW 264.7 cells. (C) 2020 Elsevier Ltd. All rights reserved.