Recovery of in vitro cotton shoots through micrografting

摘要

An efficient and highly reproducible in vitro micrografting method was developed for microshoots of cotton (Gossypium hirsutum, L.). Culture of grafted shoots in Murashige and Skoog's basal liquid medium devoid of growth regulators showed maximum rate of survival (95%), elongation of scions, number of nodes per plant and higher fresh weight compared to agar solidified medium, Rootstocks from in vitro grown decapitated etiolated seedlings were marginally better in survival percentage and growth parameters compared to light grown seedlings. Ex vitro micrografting though successful had low survival of grafted shoots (30%), minimum scion elongation and number of nodes per plant. The results demonstrate the compatibility of heterologous graft-unions (intervarietal or interspecies) in cotton. Grafted plants (70%) on transfer to greenhouse and field conditions grew to maturity and set seeds. [References: 19]