Highly selective detection of L-Phenylalanine by molecularly imprinted polymers coated Au nanoparticles via surface-enhanced Raman scattering

摘要

Molecularly imprinted film coated gold nanoprticles (MIP-AuNPs) were employed as surface-enhanced Raman scattering (SEES) substrate for sensitive and selective recognition and quantification of L-Phenylalanine (L-Phe). The MD was in-situ formed on the AuNPs by sol-gel technique using L-Phe as the template molecule, tetraethyl orthosilicate as the crosslinker and phenyltrimethoxysilane as the functional monomer. The efficient removal of template was achieved by ultrasonic treatment. The as-prepared MIP-AuNPs sensor showed a good linear relationship with the concentration of L-Phe in the range of 1.0 x 10(-8)-1.0 x 10(-4) mol L-1 with a limit of detection as low as 1.0 nmol L-1. The sensor also showed an excellent selectivity as L-Phe can be determined in the presence of other amino acid analogues, D-Phe and bovine serum. The stability of the MIP-AuNPs was manifested by the low deviation (RSD = 3.7%) for 40 days subsequent measurements.