DETECTION OF THE LCR GENE IN YERSINIA PESTIS RESPONSIBLE FOR THE RECENT OUTBREAK OF PLAGUE IN INDIA

摘要

Rapid detection of plague bacilli is particularly germane to plague epidemiology since untreated bubonic plague can rapidly progress to septicaemic oc pneumonic state. A 1.3 kb Hind-III/Xho-I DNA fragment from the plasmid pCd-1 which codes for the low Ca2+ response of Yersiniae spp. has been used to screen the isolates of Y. pestis obtained from the recent outbreak at Beed and Surat. The probe discriminated Y. pseudotuberculosis, diarrhoeagenic Escherichia coli, Klebsiella pneumoniae, Salmonella typhi and Shigella dysenteria from Y. pestis and Y. enterocolitica. Both radioactive and non-radioactive probes gave excellent result. [References: 14]