Effect of plant growth regulators on in vitro morphogenesis of Leptadenia reticulata (Retz.) W.&A. from nodal explants

摘要

Multiple shoot production was successfully achieved from axillary buds of Leptadenia reticulata using nodes as explants, and simultaneous organogenesis via callus produced from the base of the nodes. The Murashige and Skoog basal medium was supplemented with combinations of indole-3-butyric acid (IBA) and kinetin (Kn) for multiple shoot production. Maximum of six shoots were produced per node with the combination of IBA (1 mg/l) and Kn (10 mg/l) from the axillary bud. Callus produced from the base of the nodes in combination with indole-3-acetic acid and Kn was isolated for organogenesis. Organogenesis from callus was achieved with the combination of naphthaleneacetic acid (1.5 mg/l) and Kn (10 mg/l) as well as IBA (1 and 1.5 mg/l) with Kn (2 mg/l). Microshoots were transferred for rooting in IBA-containing medium, in which maximum 13 roots were produced in IBA (1 mg/l). Through sequential hardening process, well-rooted plantlets were established in the field.