Expression of anthocyanin pigmentation in wheat tissues transformed with anthocyanin regulatory genes

摘要

Screening of transgenic tissue on the basis of the anthocyanin pigmentation has been studied in whest. Cell-autonomous anthocyanin pigmentation, controlled by B and Cl anthocyanin regulatory genes under the control of constitutive CaMV35s promoter (pBC 1-7), by was obtained in scutellum of immature embryos by biolistic procedures with or without a herbicide resistance gene (pActlbar). Anthocyanin production as red/purple pigmented cells could be visualized 24 h after bombardment. Bialaphos herbicide resistant calli/plants generated transgenic sectors which showed light-dependent anthocyanin pigmeneratation. The pigmentation was suppressed in regenerating shoots but expressed in the ovary and pericarp of developing seeds. Transgenic shoots were obtained following selection of cultures cobombarded with a selectable herbicide resistance gene. Southern analysis showed that transgenes were present as multiple copy insertions in high molecular weight DNA. The results obtained showed that anthocyanin marker could be used for tracking transformed tissue on the basis of anthocyanin pigment formation whose potential is realized by environmental factors particularly light.