Molecular cloning and sequencing of a polymorphic band from rubber tree [Hevea brasiliensis (Muell.) Arg.]: the nucleotide sequence revealed partial homology with proline-specific permease gene sequence

摘要

Rubber tree (Hevea brasiliensis Muell. Arg.) is mainly cultivated in high rainfall areas (traditional). Identification of DNA marker or genes could be highly useful to develop drought-tolerant clones or screen Hevea seedling populations, which will eventually extend the rubber cultivation in non-traditional areas (under irri-gation). RAPD analysis is a valuable tool in studying genetic variation and for identification of specific gene fragments. Genomic DNAs from 37 clones were used for RAPD analysisby polymerase chain reaction (PCR), where the total and unique number of polymorphic bands varied with the clones and primers.