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Green Synthesis of Cr_2O_3 Nanoparticles Using Tridax procumbens Leaf Extract and its Antibacterial Activity on Escherichia coli

机译:Tridax procumbens叶提取物绿色合成Cr_2O_3纳米颗粒及其对大肠杆菌的抗菌活性

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摘要

Chromium oxide nanoparticles (NPs) were rapidly synthesized by reduction of potassium dichromate solution with a Tridax procumbens leaf extract containing carbohydrates as a major component which act as reducing agent. The results indicated that water-soluble carbohydrates which have aldehyde group may cause the fonnation of Cr_2O_3 nanoparticles. The purification process of the Cr_2O_3 product does not require expensive methods, since a solid product is obtained from a reaction in liquid phase. The antibacterial effect of Cr_2O_3 nanoparticles against Escherichia coli was investigated as a model for Gram-negative bacteria. Bacteriological tests were performed in Luria-Bertani (LB) medium on solid agar plates and in liquid systems supplemented with different concentrations of nanosized Cr_2O_3 particles. These particles were shown to be an effective bactericide. The resulting Cr_2O_3 nanoparticles were characterized by X-Ray Diffraction (XRD), Scanning Electron Microscopy (SEM), UV-VIS absorption and Fourier-transform infrared (FTIR) spectroscopy.
机译:氧化铬纳米粒子(NPs)通过使用含有碳水化合物作为主要成分的Tridax procumbens叶提取物还原重铬酸钾溶液而快速合成。结果表明,具有醛基的水溶性碳水化合物可能导致Cr_2O_3纳米粒子的形成。 Cr_2O_3产物的纯化过程不需要昂贵的方法,因为固体产物是从液相反应中获得的。以革兰氏阴性菌为模型,研究了Cr_2O_3纳米粒子对大肠杆菌的抗菌作用。在Luria-Bertani(LB)培养基中的固体琼脂平板上和补充了不同浓度的纳米级Cr_2O_3颗粒的液体系统中进行细菌学测试。这些颗粒被证明是有效的杀菌剂。通过X射线衍射(XRD),扫描电子显微镜(SEM),UV-VIS吸收和傅立叶变换红外(FTIR)光谱对所得的Cr_2O_3纳米颗粒进行表征。

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