首页> 外文期刊>Bioscience, Biotechnology, and Biochemistry >Identification of a New Pheromone-Binding Protein in the Antennae of a Geometrid Species and Preparation of Its Antibody to Analyze the Antennal Proteins of Moths Secreting Type II Sex Pheromone Components
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Identification of a New Pheromone-Binding Protein in the Antennae of a Geometrid Species and Preparation of Its Antibody to Analyze the Antennal Proteins of Moths Secreting Type II Sex Pheromone Components

机译:几何物种的天线中一种新的信息素结合蛋白的鉴定及其抗体的制备,以分析分泌II型性信息素成分的飞蛾的触角蛋白。

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摘要

The full-length cDNA sequence of a new pheromone-binding protein (AscrPBP2) was determined from a geometrid moth, Ascotis selenaria cretacea, which secreted a Type II sex pheromone, and an antiserum against its recombinant protein overexpressed in Escherichia coli was prepared. In addition to this antiserum against AscrPBP2, antibodies against AscrPBP1 and general odorant-binding proteins of Bombyx mori were used in Western blotting experiments to analyze the proteins in the antennae of several lepidopteran species secreting Type II sex pheromone components.
机译:从几何型蛾Ascotis selenaria cretacea分泌了II型性信息素,并测定了一种新的信息素结合蛋白(AscrPBP2)的全长cDNA序列,并制备了针对其在大肠杆菌中过表达的重组蛋白的抗血清。除了针对AscrPBP2的抗血清外,针对AscrPBP1的抗体和家蚕的一般气味结合蛋白也被用于蛋白质印迹实验,以分析几种分泌II型性信息素成分的鳞翅类动物触角中的蛋白质。

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