In vitro Evaluation of Gadolinium-Silica Mesoporous Nanoparticles-Monoclonal Antibody: Potential Nanoprobe for Prostate Cancer Cell Imaging

摘要

Purpose: The unambiguous prostate cancer diagnosis is of high global interest. Magnetic Resonance Imaging (MRI) is a low invasive modality in early stages of tumor diagnosis, especially for cancer detection. The study aim is to synthesize Gd3+ based silica mesoporous nanosphere conjugated with Monoclonal Antibody (Mab C595) and evaluate its ability as nanoprobe for prostate cancer cells detection.Method: Here, a nanoprobe, specifically recognizing in vivo MUC-1 antigen in prostate cancer cells, was synthesized and evaluated. The in vitro studies included cell toxicity, cell binding and immune reactivity assay. MR imaging parameters of this nanoprobe were investigated by MRI.Results: Results showed that this nanoprobe is a selective MUC-1 detector, without any significant cell toxicity.Conclusion: This study confirmed that this nanoprobe is potentially a selective prostate molecular imaging agent for cancer cell detection.