Synthesis and biological evaluation of 1,2,4-oxadiazole linked 1,3,4-oxadiazole derivatives as tubulin binding agents

摘要

As an aspect of our ongoing research in search of new anticancer agents, a series of novel analogs of 1,3,4-oxadiazole embedded with 1,2,4-oxadiazole moieties (11a-j) were synthesized. The structure of the final compounds was confirmed by H-1 NMR, (CNMR)-C-13 and mass spectroscopic techniques and evaluated for their in vitro anticancer activity against three human cancer cell lines (lung, breast). Among the synthesized compounds, 11 b, 11 g, 11 h, and 11i showed potent anticancer activity with IC50 values within the range of 0.34 +/- 0.025 to 2.45 +/- 0.23 mu M against three human cancer cell lines. Further, these compounds (11a-j) were investigated for molecular docking studies. Among them, compound 11 h showed strong binding affinity on binding sites of target protein EGFR (PDB ID: 4hjo) with highest docking score (-7.028). It revealed that 11 h was a strong tubulin binding agent.