A fast-response two-photon fluorescent probe for imaging endogenous H 2O 2 in living cells and tissues

摘要

AbstractAs a second messenger, hydrogen peroxide plays significant roles in numerous physiological and pathological processes and is related to various diseases including inflammatory disease, diabetes, neurodegenerative disorders, cardiovascular disease and Alzheimer's disease. Two-photon (TP) fluorescent probes reported for the detection of endogenous H2O2are rare and most have drawbacks such as slow response and low sensitivity. In this report, we demonstrate a simple H2O2-specific TP fluorescent probe (TX-HP) containing a two-photon dye 6-hydroxy-2,3,4,4a-tetrahydro-1H-xanthen-1-one (TX) on the modulation of the ICT process. The probe exhibits a rapid fluorescent response to H2O2in 9min with both high sensitivity and selectivity. The probe can detect exogenous H2O2in living cells. Furthermore, the probe is successfully utilized for imaging H2O2in liver tissues.Graphical AbstractDisplay Omitted展开▼