Construction of a Soluble Supramolecular Glutathione Peroxidase Mimic based on Host-Guest Interaction

摘要

A soluble glutathione peroxidase mimic with the optimum structure (NCD-Te-NCD_(2.2)) was con-structed based on the supramolecular host-guest interaction. The self-assembled structure and catalytic mechanism of NCD-Te-NCD_(2.2) were detailed investigation. It was proved that NCD-Te-NCD_(2.2) could form the hollow vesicle-like aggregates in water. And saturation kinetics measurement indicated that NCD-Te-NCD2.2 exhibited the typical enzyme catalytic behavior. Furthermore, benefited from the hydrophobic micro-environment in vesicle-like aggregates, NCD-Te-NCD_(2.2) exhibited the specific recognition ability for hydrophobic substrates, which was one of the important characteristics of native glutathione peroxidase. The successful construction of NCD-Te-NCD2.2 not only provides an efficient preparation method for soluble GPx mimic but also highlights for construction of other giant supramolecular biomimetic enzymes.