Towards efficient extraction of notoginseng saponins from cultured cells of Panax notoginseng

摘要

Different extraction methods including conventional organic solvent,Soxhlet,heat reflux,ultrasound-assisted and microwave-assisted extractions were attempted for efficient extraction of notoginseng saponins from dried cells of Panax notoginseng obtained in bioreactor cultures.The microwave-assisted extraction for 6 min led to a similar saponin yield (based on mg/100 mkg cells) compared to other methods that took 2-14 h.To optimize the microwave-assisted extraction conditions,the treatment time,temperature,solid/liquid (w/v) ratio and the number of repeated extraction steps were further studied.The optimal extraction method was microwave-assisted extraction for 4 min of extraction time at 50 deg C of extraction temperature (at a radiation power of 125 W) and 1:150 of the solid/liquid (w/v) ratio.The yield of total saponins extracted from P.notoginseng cells was increased with an increase of extraction steps,and it was obtained more with microwave assistance than that without microwave assistance.