Immobilization of Proteins on Highly Activated Glyoxyl Supports: Dramatic Increase of the Enzyme Stability via Multipoint Immobilization on Pre-existing Carriers

摘要

The binding of enzymes on carriers with a high degree of activation with glyoxyl groups is an excellent method for improving enzyme stability by multipoint covalent attachment on a pre-existing carrier. Glyoxyl groups are short aliphatic aldehyde groups (Support-O-CH2-CHO) that can be obtained by periodate oxidation of glyceryl groups (Support-O-CH2-CHOH-CH2OH). The unique features of glyoxyl groups are as follows: