Application of massively parallel pyrosequencing to the discovery of non-dinucleotide microsatellites: tetra- and pentanucleotide repeat markers for the Pacific abalone (Haliotis discus hannai)

摘要

The accelerated progress of ultra high-throughput DNA sequencing technologies has facilitated the process of identifying microsatellites in the genomes, attenuating laborious experimental procedures involved in traditional approaches. We applied a massively parallel pyrosequencing technique to the development of tetra- and pentanucleotide microsatellite markers for the Pacific abalone Haliotis discus hannai.