Array detection in a holographic scanning microscope

摘要

In this paper we describe a simple experimental arrangement to implement array detection in a scanning optical microscope where both the beam shaping and beam scanning are done holographically. A ferroelectric liquid crystal spatial light modulator is used to implement dynamic and reconfigurable binary holograms and an ordinary camera is used as the array detector. We use a scanning scheme that enables array detection over 120 x 120 scan positions and 40 x 40 detector array data for each position with an acquisition time of 40 s using a 15 fps (frames per second) camera. We demonstrated the working of the microscope to implement image subtraction and image scanning microscopy in both reflected and fluorescence light.