Hydrostatic fluid pressure promotes cellularity and proliferation of human dermal fibroblasts in a three-dimensional collagen gel/sponge

摘要

Cell constructs and culture systems are essential components of tissue engineering.Cell constructs are usually composed of a dense population of cells,for which long-term culture is required in vitro.However,the denser construct suffers from the absence of passive nutrient supply,gas exchange,and removal of degraded debris.We have developed a novel hydrostatic pressure/perfusion (HP/P) culture system that improves the quality of neo-tissues,providing an automated affordable system for clinical applications.We evaluated the effects of HP/P on cellularity,viability,and proliferation of human dermal fibroblasts seeded in a gel/sponge construct.HP/P and perfusion promoted cell migration and significantly increased proliferation and DNA content after 4 days culture compared to the static culture.HP/P culture is beneficial for building a denser three-dimensional fibroblast construct.