Model-based fed-batch cultivation of R.eutropha for enhanced biopolymer production

摘要

Batch kinetics of the PHB synthesis was established in bioreactor under controlled environmental conditions of pH and dissolved oxygen.The data of the batch kinetics was used to develop and identify a mathematical model.The nutrient feeding strategies in the fed-batch cultivation were developed aiming at maintenance of limiting nutrient for either growth enhancement or increased intracellular product accumulation by offline computer simulation of mathematical model.The selected fed-batch fermentation strategies,using the predicted feeding profiles,were experimentally implemented to improve the growth/product formation/productivity and establish the validity of the developed model.The model predictions were very close to the observed experimental data points.