Fed-batch production of lipase by Acinetobacter radioresistens using Tween 80 as the carbon source

摘要

Lipase production by Acinetobacter radioresistens was examined in fed-batch cultures using Tween 80 as the carbon source.An optimal medium containing 3 ml/1 Tween 80 and 7.5 g/1 yeast extract was developed in batch cultures without pH control.Assessed from the final culture pH,this composition was regarded being balanced between the carbon and nitrogen sources.Data obtained from fed-batch cultures with DO- and pH-stat feedings showed that specific growth rate mu was the intrinsic factor that determined the efficiency of lipase synthesis.The specific lipase productivity was maximal at mu - 0.1 h~(-1),while the optimal lipase production rate occurred around mu = 0.2 h~(-1) Based on a growth-associated pattern for lipase formation,the production of lipase with fed-batch culture could be simulated satisfactorily.