As a widely used neonicotinoid insecticide, thiacloprid has been observed to pose a risk to honeybees and the endocrine system of mammals. So a detection method with high sensitivity, simple operation and high throughput is required. Based on this consideration, we prepared an anti-thiacloprid monoclonal antibody (mAb, C9) and developed a fluorescence polarization immunoassay (FPIA) for the detection of thiacloprid. After optimizing the length of spacer and reaction conditions, the 50% inhibition concentration (IC50), limit of detection (LOD) and linear range (IC20 similar to IC80) of the FPIA are 15.34 ng mL(-1), 2.43 ng mL(-1) and 3.10-65.7 ng mL(-1), respectively. Meanwhile, FPIA just requires 12 min to detect the pesticide with simple operation. Then the FPIA was used to detect the thiacloprid in spiked rice, soil, cucumber and tomato samples, and recoveries were in the range of 79.1%-105.3% with 3.7%-12.3% standard deviation. The FPIA also shows good correlation with high-performance liquid chromatography for the detection of thiacloprid in tomato samples.
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机译：作为一种广泛使用的新烟碱蛋白杀虫剂，已经观察到噻虫草素对蜂窝和哺乳动物内分泌系统构成风险。因此，需要具有高灵敏度，简单操作和高吞吐量的检测方法。基于这一考虑，我们制备了抗噻丙二属单克隆抗体（MAB，C9），并为检测噻虫草的荧光偏振免疫测定（FPIA）。优化间隔物和反应条件的长度后，FPIA的50％抑制浓度（IC50），检测极限（类似于IC80）的线性范围（IC 20）为15.34 ng mL（-1），2.43ng mL（ -1）和3.10-65.7 ng ml（-1）。同时，FPIA只需要12分钟以通过简单的操作来检测农药。然后，FPIA用于检测尖米，土壤，黄瓜和番茄样品中的噻丙二属，回收率为79.1％-105.3％，标准偏差为3.7％-12.3％。 FPIA还与高效液相色谱呈良好的相关性，用于检测番茄样品中的噻虫草。
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