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Identification and evaluation of novel di- and tetranucleotide microsatellite markers from the brown bear (Ursus arctos)

机译:鉴定和评估来自棕熊的新的二核苷酸和四核苷酸微卫星标记(Ursus arctos)

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摘要

We here describe 34 novel microsatellite loci from the brown bear (Ursus arctos) identified using a next-generation sequencing approach. While we found allelic variation in 30 of these, we selected 16 polymorphic microsatellites (11 di- and 5 tetranucleotide repeats) for four multiplex-PCR assays based on genetic variability, PCR performance and interpretability. Applying these assays for the 16 novel microsatellites, we screened 31 brown bears from a northern European population. The number of alleles encountered at single loci was between 4 and 10, and expected and observed heterozygosities ranged between 0.66-0.85 and 0.60-0.97, respectively. Seven of 120 pairwise locus combinations showed linkage disequilibrium and one locus deviated from Hardy-Weinberg equilibrium, while no null-alleles were detected. Our novel multiplex-PCR assays may be useful in forensics, parentage and population genetic studies.
机译:我们在这里描述了使用下一代测序方法从棕熊(Ursus arctos)确定的34个新型微卫星基因座。虽然我们在其中30个中发现了等位基因变异,但根据遗传变异性,PCR性能和可解释性,我们选择了16个多态微卫星(11个二核苷酸和5个四核苷酸重复序列)进行四个多重PCR分析。将这些分析应用于16种新型微卫星,我们从北欧种群中筛选出31只棕熊。在单个基因座处遇到的等位基因数量在4和10之间,预期和观察到的杂合度分别在0.66-0.85和0.60-0.97之间。 120个成对基因座组合中的七个显示连锁不平衡,一个基因座偏离Hardy-Weinberg平衡,而未检测到无效等位基因。我们新颖的多重PCR分析法可能在法医,亲子关系和群体遗传研究中很有用。

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