Isolation and characterization of microsatellites in Dendrobium crystallinum and application to germplasm identification

摘要

Dendrobium crystallinum is an endangered species with good restorative and medicinal functions. To investigate the genetic diversity and structure of this species, we isolated and characterized 20 microsatellite loci derived from two microsatellite-enriched libraries. Thirty individuals from a population in Tiane were used to evaluate the genetic characteristics of the microsatellites. The numbers of alleles per locus ranged from 3 to 10 with a mean of 4.9. The observed and expected heterozygosities ranged from 0.164 to 0.602 and from 0.179 to 0.653, respectively. Transferability tests between the congeneric species and the identifications of the plantlets in the same population were successful. The 20 microsatellite loci characterized in this study will be useful for future population structure, genetic diversity and conservation genetic investigations.