Preparation of antibacterial degummed silk fiber/nano-hydroxyapatite/polylactic acid composite scaffold by degummed silk fiber loaded silver nanoparticles

摘要

In this study, an antibacterial degummed silk fiber (ADSF)nano-hydroxyapatite/polylactic acid (ADSF/nHA/PLA) porous scaffold with antibacterial properties was prepared by using degummed silk fiber (DSF) loaded with silver nano-particles (Ag NPs) as a reinforcing material. In the experiment, ADSF and nHA were used as the main variables to investigate the effect of the change of the composition ratio on the performance of the composite scaffold, and a composite scaffold with excellent performance was obtained. Firstly, the DSFs were treated with dopamine (DA) and the silver ions were reduced to Ag NPs using the strong reducibility of polydopamine (PDA) to prepare ADSF loaded with Ag NPs. Finally, ADSF/nHA/PLA composite scaffolds with antibacterial properties were prepared using ADSF as a reinforcing material. In addition, samples were found to have good mineralization capacity in in vitro mineralization experiments. At the same time, in cell culture and antibacterial experiments, ADSF/nHA/PLA scaffolds were found to have good bioactivity, biocompatibility and antibacterial properties. All the results showed that the Ag NPs loaded DSF improved the performance of the nHA/PLA composite scaffold, while the ADSF/nHA/PLA had good bioactivity and antibacterial properties, making the antibacterial ADSF/nHA/PLA composite scaffold has a great potential for bone tissue engineering.