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Xylitol production from non-detoxified corncob hemicellulose acid hydrolysate by Candida tropicalis

机译:热带假丝酵母从非排毒玉米芯半纤维素酸水解产物生产木糖醇

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摘要

The interest on use of lignocellulose for producing chemicals is increasing as these feedstocks are low cost, renewable and widespread sources of sugars. Corncob is an attractive raw material for xylitol production due to its high content of xylan. In this study, hemicellulose hydrolysate from corncobs without detoxification was used for xylitol production by Candida tropicalis CCTCC M2012462. Compared with prepared xylose medium, xylitol production with dilute acid hydrolysate medium does not seem to influence specific xylose reductase activity. The decrease in xylitol productivity with dilute acid hydrolysate medium is a result of a lower biomass concentration and lag-phase time. It appears that biomass growth rate is essential for xylitol production. In xylitol fermentation with a low initial inhibitors concentration and substrate feeding strategy, a maximal xylitol concentration of 38.8 g h~(-1) was obtained after 84 h of fermentation, giving a yield of 0.7 g g~(-1) xylose and a productivity of 0.46 g l~(-1) h~(-1).
机译:由于这些原料是糖的低成本,可再生和广泛的来源,因此使用木质纤维素生产化学品的兴趣正在增加。玉米芯由于其高含量的木聚糖而成为生产木糖醇的有吸引力的原料。在这项研究中,未经解毒的玉米芯半纤维素水解物被用于热带假丝酵母CCTCC M2012462的木糖醇生产。与制备的木糖培养基相比,用稀酸水解产物培养基生产木糖醇似乎不影响特定的木糖还原酶活性。用稀酸水解产物培养基降低木糖醇生产率是由于较低的生物质浓度和滞后时间。看来生物质生长速率对于木糖醇生产是必不可少的。在低抑制剂浓度和底物补料策略下的木糖醇发酵中,发酵84小时后木糖醇的最大浓度为38.8 gh〜(-1),木糖产量为0.7 gg〜(-1),产率为0.46 gl〜(-1)h〜(-1)。

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