A self-assembled nano-cluster complex based on cytochrome c and nation: An efficient nanostructured peroxidase

摘要

A nano-cluster complex with highly efficient peroxide activity was constructed by self-assembly of nafion (NF) and cytochrome c (Cyt c) in 50 mM phosphate buffer at 25 °C. The UV-vis spectrometry, circular dichroism (CD) and transmission electron microscopy (TEM) methods were utilized for additional characterization of the nanostructured enzyme or artificial peroxidase (AP). The nano-cluster was composed of Chain-Ball structure, with an average ball size of approximately 40 nm detected by TEM method. The Michaelis-Menten (K_m) and catalytic rate (k_(cat)) constants of the AP were obtained in 50 mM phosphate buffer solution at pH 7.0 which are equal to 2.5 ± 0.4 μ M and 0.069 ± 0.001 s~(-1) respectively. The catalytic efficiency of AP was evaluated to be 0.028 ± 0.005 μ lM~(-1) s~(-1), which was 39 ± 5% as efficient as the native HRP. The AP generated here can be used in place of HRP with high stability and activity.