Submerged culture production of chitinase by Trichoderma harzianum in stirred tank bioreactors- the influence of agitator speed

摘要

The chitinase fermentation process utilizing chitin as the sole carbon source was investigated in a strirred tank bioreactor. Agitator speed of 224 rpm was found to be msot sutable for cell growth as well as for chitinase production. Chitinase yield decreased rapidly at higher agitator speed, while decrease in cell yield a higher agitator speed was not rapid. Probably, mass transfer limitation was predominant in the fermentation process at lower agitator speed. Higher agitator speed appears to reduce chitinase production.