Optimization, antioxidant potential, modulatory effect and anti-apoptotic action in of Euphorbia bivonae polysaccharides on hydrogen peroxide-induced toxicity in human embryonic kidney cells HEK293

摘要

For the first time, we have determined the effect of solvent, liquid-solid ratio and extraction time on polysaccharides yield was evaluated using a full factorial design (23). In this present investigation, a total of 7 molecules were determined in this species. In our analysis saccharose was the dominant monosaccharides. Arabinose, pyranose, fructose, glucose, inositol, saccharose and trehalose found in E. bivonae. The results of the in vitro antioxidant assay showed that the EBPS have higher antioxidant capacity. Accordingly, the HEK293 cells pre-treated with EBPS compounds (100 mu g.mL(-1)) enhanced cell viability against H2O2 exposure. Our results revealed that H2O2-exposure induced a significant increase in intracellular ROS and lipid peroxidation in HEK293 cells. Additionally, the H2O2-induced alteration in HEK293 cells morphology, was ameliorated by EBPS treatment. In addition, EPBS pre-treated cells significantly enhanced the activities of HEK293 cells antioxidant status (SOD, CAT, GPx and GSH) that were decreased after hydrogen peroxide exposure. (C) 2018 Elsevier B.V. All rights reserved.