Purification and characterization of two novel peroxidases from the dye-decolorizing fungus Bjerkandera adusta strain CX-9

摘要

Highlights?The paper reports on two novel extracellular peroxidases fromBjerkandera adustaCX-9.?These enzymes were purified (MnP BA30 and LiP BA45) and biochemically characterized.?The molecular weights and the NH2-terminal sequences of the enzymes were determined.?Their catalytic efficiency, solvent-stability, and dye-decolorization were studied.?These enzymes may be considered as potential candidates in distaining synthetic dyes.AbstractTwo extracellular peroxidases fromBjerkandera adustastrain CX-9, namely a lignin peroxidase (called LiP BA45) and manganese peroxidase (called MnP BA30), were purified simultaneously by applying successively, ammonium sulfate precipitation-dialysis, Mono-S Sepharose anion-exchange and Sep