Sulfated polysaccharides from Rhodiola sachalinensis reduce D-gal-induced oxidative stress in NIH 3T3 cells

摘要

In this study, three sulfated polysaccharides (S-RSP1-2, S-RSP1-4 and S-RSP1-8) from Rhodiola sachalinensis were produced by chlorosulfonic acid-pyridine method. D-gal was used to develop an oxidative stress model in the mouse embryonic fibroblast cell line NIH 3T3. Effects of the three sulfated polysaccharides on D-gal-induced oxidative stress were investigated. The results showed that S-RSP1-4 improved the viability of the D-gal-induced oxidative stress in NIH 3T3 cells. The sulfated polysaccharides were found to have a better protective effect against D-gal-induced oxidative stress as compared to the native polysaccharide. Scanning electronmicroscopy also showed a significant change in the surface morphology of sulfated polysaccharides. In addition, the sulfated polysaccharides had noticeable DPPH radical-scavenging activity. In summary, our results demonstrated that D-gal was able to induce oxidative stress in NIH 3T3 cells, and sulfated group might play an important role in resistance to D-gal-induced oxidative damage. (C) 2019 Elsevier B.V. All rights reserved.