Pectic oligosaccharides hydrolyzed from citrus canning processing water by Fenton reaction and their antiproliferation potentials

摘要

Citrus canning processing water contains a valuable and renewable source of biopolymers and bioactive compounds including pectic polysaccharides. Upgrading these processing wastes can not only alleviate environmental pollution but also add value to the commodity's production. In a previous study we recovered pectic polysaccharides from citrus canning processing water. In the present study, pectic polysaccharides recycled from citrus canning processing water was depolymerized by an optimized Fenton system. The hydrolyzate was fractionated via size-exclusion chromatography into six fractions: 500 Da LMP1 3 kDa; 3 kDa LMP2 5 kDa; 5 kDa LMP3 12 kDa; 12 kDa LMP4 25 kDa; 25 kDa LMP5 100 kDa and LMP6 10 wDa. Structure analyses showed that LMP1 were homogalacturonans-enriched non-esterified polysaccharides. While LMP2 contained both HG and rhamnogalacturonan-I (RG-I). Further antitumor assay showed that in comparison with the native pectic polysaccharide with moderate antitumor activity, both LMP1 and LMP2 possessed significant antitumor activity, while the inhibitory effect of LMP1 was higher than that of LMP2, suggesting that the biological properties of LMPs was influenced by structural characteristics, including molecular weight and monosaccharide composition. (C) 2018 Published by Elsevier B.V.