Production of Acinetobacter radioresistens lipase with repeated fed-batch culture

摘要

Acinetobacter radioresistens lipase was produced with repeated fed-batch culture using a balanced medium based on Tween 80 as the carbon source.A constant cell concentration was shown to be a prerequisite to extend the number of repeated cycles,and an adequate cell growth rate was critical for a high lipase yield.The pH-stat feeding was found to sustain cell growth at a rate too high to effect the lipase production.The DO-stat feeding,on the other hand,could be manipulated to allow adequate growth rate for efficient lipase production.The lipase production rate obtained could reach 42,000 U/h in a 2.51 tank fermentor.