High Performance Liquid Chromatographic Method for Simultaneous Quantification of Naloxone and Oxycodone

摘要

High performance liquid chromatography was applied to the simultaneous determination of naloxone and oxycodone. The method depends on separation and analysis of naloxone and oxycodone using C18 analytical column (250 x 4.6 mm, 5 μm). The mobile phase consists of a mixture of potassium dihydrogen phosphate (pH 5.5; 0.1 M) and acetonitrile (70:30, v/v). The photodiode array detector was set at 210 nm. The retention times of naloxone and oxycodone were 2.893 and 4.946 min, respectively. The total run time was 6 min. The developed method was validated as per the guidelines of International Conference on Harmonization and successfully applied to the simultaneous determination of naloxone and oxycodone in their tablets. All the results of validation parameters were acceptable and confirmed that the method is appropriate for the estimation of naloxone and oxycodone simultaneously.