Simple, Rapid and Sensitive UV Spectrophotometrie Method for Determination of Temozolomide in Poly-e-caprolactone Nanoparticles

摘要

The objective of this work was to develop and validate a simple, rapid and cost effective UV spectrophotometrie method for pharmaceutical characterization of temozolomide loaded polymeric nanoparticles. The media selected was 100 mM hydrochloric acid medium (pH 1.2) and λ_(max) of temozolomide was detected at 329 nm. The method was shown to be specific, linear in the range of 3-25 μg mL~(-1) (R~2 = 0.999), accurate (recovery ranging from 99.34 to 100.90 %), precise at the intra-day and inter-day levels as reflected by the relative standard deviation values of less than 1.28 % and robust to changes in diluent media pH. The detection and quantitation limits were 0.170 and 0.510 ug mL~(-1)' respectively indicating sensitivity of the method. The method was successfully used to determine the drug content and entrapment efficiency of temozolomide loaded in poly-e-caprolactone nanoparticles.