in situ Fabrication of Biological Chitosan and Gelatin-Based Hydrogels Loading Biphasic Calcium Phosphate Nanoparticles for Bone Tissue Regeneration

摘要

Adjustably biodegradable materials have gained much attention in biomedical applications.Among of them,various hydrogel-based scaffolds have applied for regenerating soft and hard tissues.In this study,according to differently biological properties of gelatin or chitosan as well as biphasic calcium phosphate nanoparticles(BCPNPs),several injectable nanocomposite hydrogels(INgel)were enzymatically fabricated from a phenolic chitosan derivative(PCD),phenolic gelatin derivative(PGD)and BCPNPs.According the change of H2O2 concentration with follow-up the time,the in situ formation of INgel was varied from 35 to 80 s.The degradation rate of the nanocomposite materials significantly related to in presence of collagenase that expended from 3 days to over one month depending on amount of the formulated PCD.The BCPNPs-encapsulated PCD-PGD INgel enhanced mineralization in the simulated biofluid.Fluorescent cytotoxicity assay indicated that the INgel was fabricated from a higher amount of the PGD resulting in a significant proliferation of bone marrow mesenchymal stem cells.These preliminary results exhibited a great potential of the INgel for bone regeneration.