Upregulation of dentin matrix protein 1 promoter activities by core binding factor alpha 1 in human dental pulp stem cells

摘要

An osteoblast-specific transcription factor, core binding factor alpha 1 (Cbf alpha 1), is critical for osteoblast and odontoblast differentiation. In this study, the role of Cbf alpha 1 in human dentin matrix protein1 (DMP1) gene expression in human dental pulp stem cells (HDPSCs) was investigated. The desired promoter fragments were obtained and cloned into the pGL3-basic vector. It was found that Cbf alpha 1 isoforms were predominantly expressed in the cytoplasm of the HDPSCs and reached to the maximum after transfection for 48 h. Furthermore, forced overexpression of Cbf alpha 1 induced the increase of the luciferase activities of pGL3-P1-6, especially those of pGL3-P-505to+86 (p < 0.05) were the most significant. Then the site-directed mutagenesis of Cbf alpha 1 binding sites in the promoter region of nt -505 to +86 resulted in a marked decline of luciferase activities. Thus, our results suggest that Cbf alpha 1 upregulates DMP1 gene expression differentially that may contribute to the spatial-temporal expression pattern of DMP1 during odontoblast differentiation. Published by Elsevier Inc.