Preparation and In Vitro Biological Evaluation of Octacalcium Phosphate/Bioactive Glass-Chitosan/Alginate Composite Membranes Potential for Bone Guided Regeneration

摘要

The chitosan/alginate-trace element-codoped octacalcium phosphateano-sized bioactive glass (CS/ALG-teOCPBG) composite membranes were prepared by a layer-by-layer coating method for the functional requirement of guided bone regeneration (GBR). The morphology, mechanical properties and moisture content of the membranes was studied by scanning electron microscopy (SEM) observation, mechanical and swelling test. The results showed that the teOCPBG distributed uniformly in the composite membranes, and such as-prepared composite membrane exhibited an excellent tensile strength, accompanying with mechanical decay with immersion in aqueous medium. Cell culture and MTT assays showed that the surface microstructure and the ion dissolution products from teOCPBG components could enhance the cell proliferation, and especially the composite membranes was suitable for supporting the adhesion and growth behavior of human bone marrow mesenchymal stem cells (hBMSCs) in comparison with the CS/ALG pure polymer membranes. These results suggest that the new CS/ALG-teOCPBG composite membrane is highly bioactive and biodegradable, and favorable for guiding bone regeneration.