BIOTECHNOLOGICAL APPROACH TO PRODUCE CHITIN AND CHITOS AN FROM THE SHELLS OF ARTEMIA URMIANA GuNTHER, 1899 (BRANCHIOPODA, ANOSTRACA) CYSTS FROM URMIA LAKE, IRAN

摘要

Chitin and chitosan are both natural biopolymers that can be extracted from the shells (exoskele-tons) of a variety of crustaceans. The objective of the present study was to characterize the chitin and chitosan obtained from the shells of Artemia urmiana cysts and to compare these to two commercially available products purified by chemical and biological methods. The shells of Artemia cysts were obtained from Urmia Lake, Iran. We found a high yield of chitin in comparison to other sources, with a lower percentage of residual materials. We also found the efficiency of conversion of chitin to chitosan to be 20 ± 10% using the biological method described herein. The high molecular weight, crystalinity, and lower degree of deacetylation (D.D.A.) of these biopolymers obtained by the biological method were other characteristics associated with the use of Artemia urmiana cyst shells as the source. The results of analytical methods indicate that botf chitin and chitosan from empty Artemia cysts compared favourably with two commercially available products. In conclusion, we suggest that one .should consider using the biotechnological approach taken here, instead of resorting to commonly used chemical methods, in order to reduce environmental pollution butstill obtain high quality products.