The imprinting induce-fit model of specific rebinding of macromolecularly imprinted polymer microspheres

摘要

The rebinding behavior of protein-imprinted hydrogel is different from classical small molecularly imprinted polymer especially in protein aggregating state and interaction changeability between protein and imprinted sites. BSA-imprinted calcium alginate-hydroxy ethyl cellulose microspheres were prepared, and the rebinding behavior was studied. An "imprinting induce-fit model" was proposed to describe the rebinding property of protein-imprinted hydrogel. Three kinds of different interacting forms between protein and imprinted hydrogel were discovered by Scatchard analysis. Slogistic fit analysis of rebinding rate coefficient was carried out, and the imprinted hydrogel was found capable of promoting rebinding through induce-fit behavior. Higher imprinting efficiency was found in microsphere samples with lower crosslinking density. Rebinding regression equation was established, and the rebinding quantity was computable with parameters including BSA aggregate concentration [P_n], dissociating rate (k_1), and single molecule rebinding rate (k_2). The experimental and calculated rebinding concentration were compared, and errors between -9.43% and 5.59% were found.